PRINCIPLE

 

The automated procedure for the determination of Nitrite is an adaptation of the diazotization method of “Standard Methods”. Under acidic conditions nitrite ion reacts with sulfanilamide to yield a diazo compound which couples with  N-1-naphthylene-diamine dihydrochloride to form a soluble dye which is measured colorimetrically.

The color intensity is measured at 550 nm.